Expression system for hydrophobic proteins

Helen Elina helina at bio1.lan.mcgill.ca
Wed Aug 12 15:10:18 EST 1998


I am trying to express a hydrophobic protein in E. coli, but have not
succeeded in doing so.  I have tried a GST-fusion but the protein is not
recovered in the soluble fraction. 
Have any of you had success with other expression systems?  Please
advise me on this matter.
Thanks!

Helen



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