Oligo stability in water at -20 C
Richard J. Dudley
rjdudley at YAHOO.COM
Wed Aug 12 10:17:46 EST 1998
> Pure water is pH 7.0 by definition. (Equal concentration of H+ and
> OH-). If your pH meter reads 6, then it is the pH meter that is
> not necessarily the water. And I will add that most pH meters
> a precise reading in such weakly buffered conditions, so do not feel
> you have an inferior product!
The definition is true; however, Milli-Q is not "pure by definition"
water. There are dissolved gasses (e.g., CO2, N2, O2). CO2 is of
special importance, since it does play a role in buffering water (and
your blood) with the carbonate-bicarbonate buffering system. The mere
act of retrieving water from the Milli-Q tap intorduces CO2.
Adidtionally, I don't think that Milli-Q (or other deionization
systems) scrub protons; they are more geared for nitrates, sulfates,
--- --- --- -- -- -- --- --- ---
Richard J. Dudley (rdudley at iname.com)
Research Specialist V
Dept. of Cell Biology and Physiology
The University of Pittsburgh
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