DNA-Vacc...Why PBS, not TE

Wolfgang Schechinger wgschech at med.uni-tuebingen.de
Sun Aug 16 06:13:01 EST 1998


Ho Bjorn, 

I'd assume that TE will be toxic to the mouse. Tris is not very 
physiological and EDTA will complex calcium ions in body fluids. 
While PBS is " physiological" meaning all compounds will be found in 
the body. And PBS should be quite physiological regarding salt 
concentration. TE is not.

Wolfgang
> Hi,
> 
> Why should anyone use PBS to solute the plasmid before injection
> into a mouse and not TE-buffer?
> 
> Thanks in advance,
> 
> Chris
> 
> Answer to Christoph.Hotz-Behofsits at mbi.uib.no
> 
> 
> 
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Wolfgang Schechinger         
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de * wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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