DNA-Vacc...Why PBS, not TE

Cornelius Krasel krasel at wpxx02.toxi.uni-wuerzburg.de
Sun Aug 16 12:01:06 EST 1998


Bjørn Olav Kvamme <Bjorn.Kvamme at mbi.uib.no> wrote:
> Why should anyone use PBS to solute the plasmid before injection into a
> mouse and not TE-buffer?

TE is hypotonic, i.e. you will probably lyse a few million erythrocytes
if you don't inject your plasmid *very* slowly. Furthermore, EDTA complexes
Calcium which doesn't sound too healthy either. I have no idea about the
toxicity of injected Tris.

--Cornelius.

-- 
/* Cornelius Krasel, U Wuerzburg, Dept. of Pharmacology, Versbacher Str. 9 */
/* D-97078 Wuerzburg, Germany   email: phak004 at rzbox.uni-wuerzburg.de  SP4 */
/* "Science is the game we play with God to find out what His rules are."  */



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