blunt ligation problem

medbh at biovax.leeds.ac.uk medbh at biovax.leeds.ac.uk
Mon Aug 17 10:57:45 EST 1998


In answer to queries:  1.  To generate a blunt ended plasmid, I cut it with SmaI.  2.  I did also dephosphatase the plasmid.  This worked as I tested this by comparing normal digested plasmid to dephosphatased plasmid when transforming the bacteria.



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