Thrombin Cleavage of GST Fusions

Michael DiDonato michael.didonato at utoronto.ca
Mon Aug 17 13:09:41 EST 1998


Does anyone know of a way to optimize thrombin cleavage of GST fusion such
that only the five base site is cleaved and not the three base sites.  I
am getting the majority of cleavage at the five base site but also some
annoying cleavage at the three base sites present in my protein.  I have
heard of using heparin in the cleavage reaction, does anyone know of this?

Any help would be appreciated.

Mike

-- 
Michael DiDonato
Structural Biology and Biochemistry
The Hospital for Sick Children
Toronto, Ontario, Canada



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