Degradation of DNA during EcoRI restriction

Kajetan Groicher groi9663 at
Mon Aug 17 17:44:01 EST 1998


I have had a very similar problem with Eco RI (eg, smearing).  The
problem turned out to be too much glycerol.  I presumed the smear to be
the result of extensive Star activity (Eco RI is known to exhibit star
activity in glycerol [] above 5%.

I agree with John's suggestion.

Best of Luck-

More information about the Methods mailing list