Degradation of DNA during EcoRI restriction

Kajetan Groicher groi9663 at uidaho.edu
Mon Aug 17 17:44:01 EST 1998


Frederik-

I have had a very similar problem with Eco RI (eg, smearing).  The
problem turned out to be too much glycerol.  I presumed the smear to be
the result of extensive Star activity (Eco RI is known to exhibit star
activity in glycerol [] above 5%.

I agree with John's suggestion.

Best of Luck-
Kajetan



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