DNA-Vacc...Why PBS, not TE

Patrick F.H. Lai pfhlai at LOOKSMART.COM
Tue Aug 18 20:25:54 EST 1998


---- Begin Original Message ----
Hi,
Why should anyone use PBS to solute the plasmid before injection into a mouse and not TE-buffer?
Thanks in advance,
Chris

Answer to Christoph.Hotz-Behofsits at mbi.uib.no
---- End Original Message ----

Dear Chris,

IMHO, cells do not like Tris nor EDTA.
THese things are good for storing your DNA, but PBS is more agreable
for cells, I suppose.
I know of a few enzymes that can be inhibited by Tris (e.g. sucrase).
EDTA will mess up the Calcium metabolism, almost for sure.
Also, TE is usually used for DNA at pH=8 to 8.5, which is too high.
PBS at about pH 7.something is closer to what cells like.

Just a thought......





Hope this is useful info.   :-)


Patrick F.H. Lai  < PFHLai at looksmart.com >
Graduate Student
University of Toronto
Toronto, Ontario, Canada





       
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