Remove endotoxin?

Richard Near rocket1 at bu.edu
Thu Aug 20 21:45:19 EST 1998


I have recently had low efficiency transfections using plamids made
by alkaline lysis /phenol extraction/ and PEG precipitation. I saw
a blurb from Quagen suggesting that endotoxin contamination may be
a significant contributor to such low efficiency. I grow my plasmids
in end- bugs. Aside from this (and purchasing a relatively expensive
kit ) , is there a (or some) ways to remove endotoxin??

Rick
rocket1 at bu.edu

Thanks




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