8 Azaguanine selection - how long ?

[Ina Hinners]

Jens Rainer Hansen wrote:
> 
> I have some SP2 myeloma cells, they are not all HAT sensitive. Therefore I
> want to select for the HAT sensitive by using 8 Azaguanine. Do you know how
> long I have to grow the SP2 cells in 8-azaguanine ?
> 
> Yours Jens Rainer Hansen


Dear Jens, I do the selection for 2-3 weeks, after this period all HAT
insensitive myelomas should be dead.
good luck, Ina

-- 
Ina Hinners
INRA
Laboratoires de Biologie Moleculaire et Cellulaire
Unite des Transports Cellulaires
78352 Jouy-en Josas
France
Tel.: +33(0)134652548
Fax.: +33(0)134652241
email: hinners at biotec.jouy.inra.fr