8 Azaguanine selection - how long ?

Ina Hinners hinners at biotec.jouy.inra.fr
Thu Aug 20 04:38:10 EST 1998

Jens Rainer Hansen wrote:
> I have some SP2 myeloma cells, they are not all HAT sensitive. Therefore I
> want to select for the HAT sensitive by using 8 Azaguanine. Do you know how
> long I have to grow the SP2 cells in 8-azaguanine ?
> Yours Jens Rainer Hansen

Dear Jens, I do the selection for 2-3 weeks, after this period all HAT
insensitive myelomas should be dead.
good luck, Ina

Ina Hinners
Laboratoires de Biologie Moleculaire et Cellulaire
Unite des Transports Cellulaires
78352 Jouy-en Josas
Tel.: +33(0)134652548
Fax.: +33(0)134652241
email: hinners at biotec.jouy.inra.fr

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