deaerate or not deaerate

Zbigniew Rudzki mprudzki at
Thu Aug 20 09:20:03 EST 1998

Does degasing of polyacrylamide belong to the group of totally magic and
irrational procedures contaminating molecular biology ?
Today, forced by the technical reasons, I tried for the first time to skip
it and I added twice as much TEMED as usually (20 uL per 70 mL).  It
resulted in an almost instant catastrophic polymerization :)

So what is your opinion on degasing, after all rather troublesome a
procedure ?

Zbigniew Rudzki
Department of Pathology
Jagiellonian University
Krakow, Poland

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