deaerate or not deaerate

Jongrak Kittiworakarn g3937506 at STUDENT2.MAHIDOL.AC.TH
Fri Aug 21 03:05:54 EST 1998


> Re: deaerate or not deaerate
>
> (rjdudley at yahoo.com)
> 20 Aug 1998 17:58:56 -0700
>
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> To: methods at net.bio.net
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> From: rjdudley at yahoo.com ("Richard J. Dudley")
>
> Subject: Re: deaerate or not deaerate
>
> Date: 20 Aug 1998 17:58:56 -0700
>
> I usually do. I have had problems that I can directly attribute to
> not degassing the acrylamide. Usually for tris-tricine gels of high
> percentage. I haven't had any trouble yet with SDS-PAGE or sequencing
> gels, but I have made it a habit to degas because of troubles with
> other gels. it only takes a monute or so anyway.

I worked with tris-tricine gels. I have never degased the acrylamide solution,
and it worked O.K. for me. The only problems I had with the gel is that the
gel crack on drying.


Jongrak




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