to deaerate or not to deaerate

Greg Hawkins greg.hawkins at epicentre.com
Fri Aug 21 08:45:11 EST 1998


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As far as gas added to acrylamide to inhibit polymerizaton, I recently =
had a problem with Fisher pre-made 40% 19:1.  It would not polymerize, =
but I was not degasing.  I was filtering through a syringe filter.  The =
same solution worked at my old job, but we were making a 6% mix in bulk =
and filtering with vacuum.  The filtering automatically degassed the =
mix.  Maybe the problem is solved.  I have an experiment to run.......

Gregory A. Hawkins, Ph.D.
Senior Scientist Genetic Analysis
Epicentre Technologies
1202 Ann Street
Madison, WI 53713
Phone:  (608) 258-3090 ext. 233
Fax:       (608) 258-3099
Email:  greg.hawkins at epicentre.com

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<DIV><FONT size=3D2>As far as gas added to acrylamide to inhibit =
polymerizaton, I=20
recently had a problem with Fisher pre-made 40% 19:1.&nbsp; It would not =

polymerize, but I was not degasing.&nbsp; I was filtering through a =
syringe=20
filter.&nbsp; The same solution worked at my old job, but we were making =
a 6%=20
mix in bulk and filtering with vacuum.&nbsp; The filtering automatically =

degassed the mix.&nbsp; Maybe the problem is solved.&nbsp; I have an =
experiment=20
to run.......</FONT></DIV>
<DIV><FONT size=3D2></FONT>&nbsp;</DIV>
<DIV><FONT color=3D#000000 size=3D2>Gregory A. Hawkins, Ph.D.<BR>Senior =
Scientist=20
Genetic Analysis<BR>Epicentre Technologies<BR>1202 Ann =
Street<BR>Madison, WI=20
53713<BR>Phone:&nbsp; (608) 258-3090 ext.=20
233<BR>Fax:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; (608) =
258-3099<BR>Email:&nbsp;=20
<A=20
href=3D"mailto:greg.hawkins at epicentre.com">greg.hawkins at epicentre.com</A>=
</FONT></DIV></BODY></HTML>

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