western blot of basic proteins

Marianne Ostermayer ostermayerm at ubaclu.unibas.ch
Fri Aug 21 08:34:05 EST 1998


One of my recombinant His-tagged protozoen protein (45kDa), expressed in
E. coli, does not work in western transfers, perhaps because it has an
isoelectric point of 9,6.  I do semi dry transfers and tried several
transfer buffers, but the results were disappointing. The protein was
transfered less than 50%. Does anybody have an idea, how to blot a basic
protein like this?

Thanks a lot
Andrea

Andrea Irion, Swiss Tropical Institute, Socinstr. 57, CH-4002 Basel
IRION at ubaclu.unibas.ch




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