ghendo at ozemail.com.au
Fri Aug 21 23:10:51 EST 1998
Have you tried using Chelex 100 from Bio-Rad? It is a simple procedure.
Guy A. Hoelzer wrote in message ...
>In a recent issue of Science (vol. 281:402-406), Poinar et al. reported
>that use of a novel reagent (N-phenacylthiazolium) greatly improved the
>efficacy of DNA extraction from ancient dung samples. I have been unable
>to find an email address for either Poinar or Paabo to ask about this
>reagent, so I would appreciate email addresses for either of them. My
>question is whether this reagent improves DNA extraction from relatively
>fresh feces, as well. It apparently breaks "cross-links between reducing
>sugars and amino groups", thus freeing the DNA; but the article does not
>discuss when these cross-links form. I am also interested in whether this
>chemical is commercially available.
>Guy Hoelzer e-mail: hoelzer at med.unr.edu
>Department of Biology phone: 702-784-4860
>University of Nevada Reno fax: 702-784-1302
>Reno, NV 89557
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