lambda-ZAP lifting

[Jorge Muschietti]

Hi,

did anybody try to do more than one lifting for a lamda-ZAP cDNA expression
library? 
maybe, incubating the plates with the plaques 6 hrs at 37 with  the wet (in
IPTG solution) nitrocellulose filter and then a 2nd lifting ON?

thanks
Jorge

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Jorge P. Muschietti
Plant Gene Expression Center
University of California-Berkeley
USDA-ARS
800 Buchanan St.
Albany, CA 94710
TE: 	1 (510) 559-5918
FAX:	1 (510) 559-5678
e-mail: prometeo at nature.berkeley.edu
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