to deaerate or not to deaerate

Beth Wapelhorst weazel at animal.blarg.net
Sun Aug 23 00:27:58 EST 1998


I never filter and I never degas. Any non-polymerization problem I've ever
had was caused by stale ammonium persulfate. 

just somethin' else for you to check :)

beth

>As far as gas added to acrylamide to inhibit polymerizaton, I recently =
>had a problem with Fisher pre-made 40% 19:1.  It would not polymerize, =
>but I was not degasing.  I was filtering through a syringe filter.  The =
>same solution worked at my old job, but we were making a 6% mix in bulk =
>and filtering with vacuum.  The filtering automatically degassed the =
>mix.  Maybe the problem is solved.  I have an experiment to run.......

>Gregory A. Hawkins, Ph.D.
>Senior Scientist Genetic Analysis
>Epicentre Technologies
>1202 Ann Street
>Madison, WI 53713
>Phone:  (608) 258-3090 ext. 233
>Fax:       (608) 258-3099
>Email:  greg.hawkins at epicentre.com

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><DIV><FONT size=3D2>As far as gas added to acrylamide to inhibit =
>polymerizaton, I=20
>recently had a problem with Fisher pre-made 40% 19:1.&nbsp; It would not =

>polymerize, but I was not degasing.&nbsp; I was filtering through a =
>syringe=20
>filter.&nbsp; The same solution worked at my old job, but we were making =
>a 6%=20
>mix in bulk and filtering with vacuum.&nbsp; The filtering automatically =

>degassed the mix.&nbsp; Maybe the problem is solved.&nbsp; I have an =
>experiment=20
>to run.......</FONT></DIV>
><DIV><FONT size=3D2></FONT>&nbsp;</DIV>
><DIV><FONT color=3D#000000 size=3D2>Gregory A. Hawkins, Ph.D.<BR>Senior =
>Scientist=20
>Genetic Analysis<BR>Epicentre Technologies<BR>1202 Ann =
>Street<BR>Madison, WI=20
>53713<BR>Phone:&nbsp; (608) 258-3090 ext.=20
>233<BR>Fax:&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; (608) =
>258-3099<BR>Email:&nbsp;=20
><A=20
>href=3D"mailto:greg.hawkins at epicentre.com">greg.hawkins at epicentre.com</A>=
></FONT></DIV></BODY></HTML>

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-- 
sugar beth wapelhorst              "I don't think I'm alone when I say I'd
weazel at blarg.net                    like to see more and more planets fall
www.blarg.net/~weazel               under the ruthless domination of our
                                    solar system." - Jack Handey



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