Trouble with SOLR Cells
awarkman at julian.uwo.ca
Mon Aug 24 08:10:14 EST 1998
In article <see_sig-2408981303120001 at 192.168.0.84>, rgrant at netscape.net wrote:
>Has anyone out here had experience with Stratagene's SOLR cells (E. coli)?
>We find (from some clones that we were given) that they're very clumpy
>after overnight growth, and although they pellet nicely enough, they do
>not resuspend very well when doing minipreps.
I believe that the SOLR cell are generally only used (at least in our lab) to
rescue the phagmid (i.e. pBluescript) during the "in vivo excision" procedure
of library screening. We routinely retransform our rescued phagmid into
XL1-Blue MRF' cells (E. coli) due to the poor quality plasmid preps obtained
with SOLR cells.
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