Cycloheximide selection in yeast-2-hybrid

Miguel Aguilera maguilera at
Mon Aug 24 13:26:22 EST 1998

Hello everybody!

Did anybody use the cycloheximide dominant selection marker in pAS2-1 of
the Matchmaker-2 kit? Does it work in the first generation or several
passes are needed to recover the colonies that lost this plasmid?

Any comment is welcome! 

Thanks for your help,



Miguel Aguilera
Instituto Cajal, CSIC
Madrid - SPAIN

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