Cycloheximide selection in yeast-2-hybrid

Miguel Aguilera maguilera at iib.uam.es
Mon Aug 24 13:26:22 EST 1998


Hello everybody!

Did anybody use the cycloheximide dominant selection marker in pAS2-1 of
the Matchmaker-2 kit? Does it work in the first generation or several
passes are needed to recover the colonies that lost this plasmid?

Any comment is welcome! 

Thanks for your help,


Miguel


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Miguel Aguilera
Instituto Cajal, CSIC
Madrid - SPAIN
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