Which should be CIPed, 7K one or 3K one?

Yang Sheng shengyang at USA.NET
Mon Aug 24 22:59:14 EST 1998


Hi, all!

	I am to link a 3K fragment with a 7k one. They were cut out by ScaI from two plasmids 
derived from pBluscript SK(+). I considered that one should treated with CIP to prevent it from 
self-circlized. So, which should be CIPed?

Thank you for your advice.
ATGCGTTTTAAACCGTTTGTAACTGTTTTCTTCGACTGAAACTGGACTGGATCATGAAGCTGTAG
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ATGGCAAATCCGTATGCGTTTTAAACCGTTTGTAACTGTTTTCTTCGACTGAAACTGGACTGGATC




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