[ANNOUNCE] CloneIt Finding cloning strategies
lindenb at jouy.inra.fr
Tue Aug 25 02:26:37 EST 1998
: : _________________________________________________________________
: : CLONEIT
: : AN ANSI-C PROGRAM FINDING SUB-CLONING STRATEGIES, IN-FRAME DELETIONS AND
: : FRAMESHIFTS USING RESTRICTION ENZYMES AND DNA POLYMERASES.
: (i) The example sequence and other files cited on the web page are not
: in standard Unix format, i.e., they have some weird end-of-line
: markers, so you have to hack them to make the files readable on Unix
: systems or the program hangs. Surely it's better to reformat the
: linked files in standard Unix format at the web page end?
Thank you for your post. I'm currently writing a new version of
CloneIt which will have a few bugs (Sorry about this !) which
will be able to handle more files format (including binary files
from DNA STrider), to browse the strategies (go next, previous..)
: (ii) I tried using the "project" file input as described on the web
: page. Got a segmentation fault at the line for "set.vector.atg".
Thank you ! I'll correct this in the new version.
I don't use a lot the "project" option ( this was a wish of
one of my reviewers)
: Correct that and 9 other similar lines and the project file "set.*"
: statements now work properly.
: (iv) The documentation does not make it very clear how you use the
: program to clone a random section of plasmid A into a random section of
: plasmid B.
The program finds the polylinkers boundaries using the small provided
database called "Polylinkers.Set".
It can be worked out, but more examples would be a great
: help. Otherwise people will think it can only be used for specialised
: two-hybrid system type subcloning.
And It's not ! :-)
: Having said that, I was pleased to see that in the first example of
: subcloning with plasmids of mine that I gave it, the strategy first
: suggested was the one I'd found the hard way myself.
Thank you for this advertising !
: Best wishes,
: Chris Boyd PhD MRC Human Genetics Unit, Western General Hospital
: Christopher.Boyd at hgu.mrc.ac.uk Crewe Road, Edinburgh EH4 2XU, Scotland
: http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd/ T/F: +44(0)131 3322471/3432620
6098)o%::%o9 Pierre LINDENBAUM
098)o%::::::%o9 Biologie Moleculaire des Rotavirus
6o%::::::%o(860 Unite de Virologie et Immunologie Moleculaires
6o%::%o(8609 Institut National de la Recherche Agronomique
o(86098) 78352 JOUY-EN-JOSAS CEDEX
098)o%:::%o(8609 Tel: 33-(0)1-34-65-26-05
6o%:%o(86098) Fax: 33-(0)1-34-65-26-21
CloneIt WWW Home Page:http://locus.jouy.inra.fr/soft/cloneit/cloneit.html
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