problems digesting maxi-prep DNA???

mattboy2 at yahoo.com mattboy2 at yahoo.com
Tue Aug 25 08:37:14 EST 1998


has anyone ever had problems digesting plasmid DNA form a maxi-prep, when the
mini-prep DNA cut fine??  I'm using the Guanidine Thicyanate method to
extract the DNA, in both cases.  The only difference I can see is the scale,
obviously, and the way I wash the diatomaceous earth of Guanidine (mini-prep
= centrifugation, maxi-prep = vacuum).

cheers,

matt

mattboy2 at yahoo.com

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