Which should be CIPed, 7K one or 3K one?
weazel at animal.blarg.net
Tue Aug 25 20:42:29 EST 1998
phosphatase the one that's got the drug resistant gene in it.
shengyang at USA.NET (Yang Sheng) writes:
> I am to link a 3K fragment with a 7k one. They were cut out by ScaI from two plasmids
>derived from pBluscript SK(+). I considered that one should treated with CIP to prevent it from
>self-circlized. So, which should be CIPed?
>Thank you for your advice.
> _____ ________ _______ _______ _ Yang Sheng
> __ / / /_ __ `/_ __ \__ __ `/ Shanghai Inst. of Biochem.
> _ /_/ / / /_/ /_ / / /_ /_/ / Academia Sinica,320 Yueyang Rd.
> _\__, / \__,_/ /_/ /_/ _\__, / Shanghai 200031,P.R.China
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