Need help

Ahmet Arman aa374885 at OAK.CATS.OHIOU.EDU
Tue Aug 25 19:31:53 EST 1998


Hi netters,
I am proposing one experiment but I need some information about this. 

I will select Single stranded DNAs for one protein (protein is about 22000
da) using SELEX technique. 
My idea:
____Take SSDNAs and protein mix up and incubate half hours.
____ filter protein-DNA solution
____wash filter
____isolate DNAs which attached to protein from filter
____make PCR with biotinylated primer
____after 7-10 selection
____clone DNAs which affinity to protein


Question:
a) Which filter will I use for this experiment?

Protein should stick column but free oligonucleotides should go through
column.

b) How can I isolate DNA which attached to protein from filter.


I checked all journals for references bu I could not find it.

Thank you for your cooperation.


Ahmet Arman
aa374885 at oak.cats.ohiou.edu






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