aa374885 at OAK.CATS.OHIOU.EDU
Tue Aug 25 19:31:53 EST 1998
I am proposing one experiment but I need some information about this.
I will select Single stranded DNAs for one protein (protein is about 22000
da) using SELEX technique.
____Take SSDNAs and protein mix up and incubate half hours.
____ filter protein-DNA solution
____isolate DNAs which attached to protein from filter
____make PCR with biotinylated primer
____after 7-10 selection
____clone DNAs which affinity to protein
a) Which filter will I use for this experiment?
Protein should stick column but free oligonucleotides should go through
b) How can I isolate DNA which attached to protein from filter.
I checked all journals for references bu I could not find it.
Thank you for your cooperation.
aa374885 at oak.cats.ohiou.edu
More information about the Methods