peak at 270nm

oliver.kudlacek at univie.ac.at oliver.kudlacek at univie.ac.at
Tue Aug 25 11:12:27 EST 1998


I have a problem with an experiment as simple as a boiling prep.
Scanning the DNA after the boiling prep I get a Peak at 270nm, but no
at 260nm.
Sambrook and Maniatis suggest a contamination with Proteins.
OK; but I can't get rid of this Peak even after repeated
Phenol/Chloroform extractions.
There is Plasmid DNA in the prep and I can digest it well.
But there a Problems whenn I try to run a PCR.

What is this Peak?? Where does it come from?? What can I do to get
clean DNA??

Thanks in advance

Oliver

oliver.kudlacek at univie.ac.at



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