bacterial transformation

Mona Damaj mdamaj at bio1.lan.mcgill.ca
Wed Aug 26 15:11:54 EST 1998


Hello,

We are facing problems in tranforming Acetobacterium (a gram-positive
bacterium). We tried the electroporation of the cells, but it did not
work. We then tried to remove the cell wall by lysozyme digestion before
electroporation, but it did not work. We are now thinking of partially
digesting the cell wall.

Any helpful suggestions or protocols!

Thanks.



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