assay for beta-galactosidase

mattboy2 at yahoo.com mattboy2 at yahoo.com
Wed Aug 26 07:54:20 EST 1998


hard to do beta-gal assay in suspension cells, I would use GFP (green
fluorescent protein), which doesn't need a susbstrate.	Of course, you do ned
access to a flow cytometer.  But it does work for suspension cells, really
well.

matt

In article <6s0ilj$jtj$1 at nnrp1.dejanews.com>,
  marc.bolliger at bc.biol.ethz.ch wrote:
> Hello
>
> I am using the Drosophila Expression System from Invitrogen. For a positive
> control of transfection into Schneider S2 cells, I will use the beta-
> galactosidase assay. Does somebody has a protocol for cells in suspension?
>
> Regards,
> Marc Bolliger
> Lab for Biochemistry I
> ETH Zuerich
> Switzerland
>
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