blunt ligation problems

Jim Graham graham at biodec.wustl.edu_nospammmmmmm
Wed Aug 26 16:43:50 EST 1998


> If you used Taq to make the PCR products, they will leave hanging A's 
> are NOT blunt product.. you would have to digest

Oh, but some 70% of the ends generated by Taq polymerase are indeed
blunt. The basis for their greatly reduced ligatability has not been
adequately explained. (A general reduction in ligation efficiency
following PCR is widespread across all sorts of different techniques
which try to get around the basic observation. This has been an
unresolved problem with PCR from day one.)

Anyone?

(E-mail copies pleeeease)

Jim
J. Graham PhD
Biology Department



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