Primers:bigger=better?

David Hills david.hills at bbsrc.ac.uk
Thu Aug 27 11:10:14 EST 1998


Will my PCRs improve if I use bigger primers so that I can go for higher 
Tm.s etc? This is on genomic DNA. 21-mers give a few bands would , say, 
28-mers give better results for same target?
Cheers,
Dave




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