Mechanism of luciferase degradation?

Bernard P. Murray, PhD bpmurray*STUFFER* at socrates.ucsf.edu
Thu Aug 27 10:51:25 EST 1998


Hello all,
     The subject line pretty much sums up the question - How is
firefly luciferase degraded?  Lysosomes?  Proteasome?  Ubiquitination?
I am primarily interested in the pathway(s) used during expression
in S.cerevisiae but any answer should prove helpful.  I didn't see
anything appropriate using Medline.

     Okay, while I am about it, is anything known about the
degradation of other common reporters? - CAT, beta-gal,
unfused GFP etc.

     Thanks for any hints,
          Bernard
-- 
Bernard P. Murray, PhD
Dept. Cell. Mol. Pharmacol., UCSF, San Francisco, USA



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