Cracking Preps for identifying inserts

Arthur Wohlwill U55850 at uic.edu
Thu Aug 27 12:10:03 EST 1998


Does anyone recall a "cracking prep" protocol for identifying inserts into 
plasmids?  One took colonies, incubated them in some buffer under heat and ran 
some on a gel.  It was possible to identify medium to inserts this way.  I 
need the protocol details.  
Thanks,

Arthur Wohlwill adwohlwi at UIC.EDU



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