Which should be CIPed, 7K one or 3K one?
gerdn at ibg.uit.no
Wed Aug 26 01:57:00 EST 1998
> The reason why did I consider that one of the fragments should be CIPed is
> that I want to increasing the effiency of the ligation. 3k + 7k => 10k. It
> is said that the reaction is difficult. The product is too large, more, the
> fragments are blunt-ended and it is easier for they to re-circularized.
There is a chapter in Maniatis (Sambrock., et al) and probably also in
other common laboratory manuals, dealing with the problem of inter- /
intramolecular ligation. There you should be able to find out how to
increase the ligation efficiency, and it would also help you decide
which one to CIP.
More information about the Methods