digitonin stock solution

Johannes Winkler nospam_uzs01b at uni-bonn.de
Fri Aug 28 08:02:12 EST 1998


I presume you are having difficulties dissolving the digitonin?
Dissolving 1% digitonin in TBS (without the EDTA) works fine in a microwave 
or you can warm it in a waterbath to 37 deg. (takes ages and doesn't give 
better results in my hands, though).

hjaeck wrote:
> Does someone have a good protocol to make a 1% digitonin solution in
> 150mM NaCl/50mM Tris pH=8.0/1mM EDTA?

Johannes Winkler
Dept. of Immunology
University of Bonn
Roemerstr. 164
53117 Bonn

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