Self-made TOPO cloning

Marcus Wernitz viacx at
Fri Aug 28 03:21:50 EST 1998

Has anybody thought of a self-made TOPO-like-cloning Kit?

It should not be a problem to sythesize and ligate the
EcoRI-EcoRI-Fragment with the AflI-site and the
topoisomerase-binding-sites into any plasmid at hand. 

But does the buyable calf-thymus topoisomerase bind to the binding sites
like it was originally described for the Vaccinia topoisomerase?

Or migth there be a mojor problem in transformation of the competent
cells. like DH10beta?

Who is having any good ideas about this?



Marcus Wernitz
Martin-Luther-Universitaet Halle-Wittenberg
Landwirtschaftliche Fakultaet
Institut fuer Pflanzenzuechtung und Pflanzenschutz
Lehrstuhl fuer Phytopathologie und Pflanzenschutz
Ludwig-Wucherer-Str. 2
06108 Halle a.d.Saale

Tel.: +49 (0)345-5522666	Fax : +49 (0)345-5527120
E-Mail: Wernitz at

06114 Halle a.d.Saale
Tel.: +49 (0)345-2906800

NEU: Halle Reise-/ Kneipenfuehrer unter:

More information about the Methods mailing list