Best protocol to prepare BAC DNA

daniel jacobs d.jacobs at
Sun Aug 30 23:37:22 EST 1998

In article <l03102802b20a480453b4@[]>, jaikawa at UCSD.EDU
(Jun-Ichi Aikawa) wrote:

> I heve tried to prepare BAC DNA from 500ml E.coli culture using QIAGEN
> column and obtained 20ug DNA. When I cut it, looks like genomic DNA,
> showing no clear DNA digestion patten. HAve you ever prepared BAC DNA? What
> is best protocol?
> Thanks.
> Jun-ichi Aikawa, Ph.D.
> University of California, San Diego

Dear Jun-ichi

I hate to mention work I have published but have a look at the April 1998
issue of Biotechniques, it might be what you are looking for.


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