Peak at 270nm

oliver.kudlacek at univie.ac.at oliver.kudlacek at univie.ac.at
Sun Aug 30 03:21:40 EST 1998


I have a problem with a method as simple as a boiling prep of plasmid
DNA.
Scanning the DNA after the prep I get a peak at 270 nm but no at
260nm.
Sambrook and Maniatis suggest a contamination with proteins.
OK But I cant get rid of this Peak even after repeated
Phenol/Chloroform Extractions.
 There is plasmid DNA in the prep and I can digest it well.
But there a problems with PCR.

What is this Peak, where does it come from and how can I get rid of it
and get clean DNA???

Thanks
Oliver

Oliver.Kudlacek at univie.ac.at



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