Q:BIG Dye term

tom chappell t.chappell at ucl.ac.uk
Sun Aug 30 14:42:31 EST 1998


Joseph C. Bagshaw <jbagshaw at wpi.edu> wrote:

> I have an ABI 310 and I've recently switched to the Big Dye terminator
> chemistry.  It sucks swamp water.  The results I get are the worst I've
> ever seen, including the kit controls.  I conclude that (a) Big Dye
> terminators don't work on the 310, or (b) Big Dye terminators don't work,
> period.
> 
> ********************  HAVE GENES, WILL TRAVEL  ********************
> Joe Bagshaw, Worcester Polytechnic Institute
> jbagshaw at wpi.edu
> Roadkill on the information superhighway.
> 
For direct sequencing of PCR products, I have nover seen a kit that
works as well as Big Dye terminators. Beautiful, even, intense peaks out
at 400 bp on an ABI 310.




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