Qiaex II Gel Extraction Kit

Beat beat.reidy at ipw.agrl.ethz.ch
Mon Aug 31 10:44:15 EST 1998


Dear all,
I am using Qiaex II Gel Extraction Kit to extract a very well amplified 500bp 
PCR product from agarose gels. Using special agarose (SEAKEM GTG), did not 
overdry the pellet, and was using TE buffer pH 8.0 (pH of MiliQ is too low) to 
redissolve the DNA...  changed all variables in the system...
However, final DNA yield was always very low  (aprox 7ng/ul), miles away from 
the 60%  efficiency given by Qiagen.
Does somebody have a solution to this problem? 

I appreciate your help!
   



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