Qiaex II Gel Extraction Kit
beat.reidy at ipw.agrl.ethz.ch
Mon Aug 31 10:44:15 EST 1998
I am using Qiaex II Gel Extraction Kit to extract a very well amplified 500bp
PCR product from agarose gels. Using special agarose (SEAKEM GTG), did not
overdry the pellet, and was using TE buffer pH 8.0 (pH of MiliQ is too low) to
redissolve the DNA... changed all variables in the system...
However, final DNA yield was always very low (aprox 7ng/ul), miles away from
the 60% efficiency given by Qiagen.
Does somebody have a solution to this problem?
I appreciate your help!
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