KEvans at lifetech.com
Mon Aug 31 10:05:23 EST 1998
>From: "Tam Anh Le" <leta at svhm.org.au>
>Subject: Lipfectamine fluorescent
>Date: Mon, 31 Aug 1998 04:46:47 +1000
>I've just started using lipofectamine from Gibco (life technologies) to
>transfect mouse Rho0 cells, however in the initial experiments I used a
>pGreen plasmid ( which codes a for a fluorescent protein). I found in the
>control of Rho0 cells and lipofectamine only that fluorescent activity was
>quiet intense in comparison to my autofluorescent control ( ie Rho0 cells
>only). My question is that although the intensity of the combination of
>pGreen plasmid with lipofectamine was higher than all controls :
>1. has anyone notice the same thing with this particular transfecting
>2. is there a way of eliminating this problem of fluorescent caused by the
>Tam Anh Le
>St Vincent Hospital Melbourne.
Various cationic lipids frequently exhibit a white to orange color
fluorescence when added to cells. DMRIE-C transfection reagent has a low
background fluorescence with FITC conditions; much lower fluorescence than
you are seeing with LipofectAMINE.
Life Technologies, Inc.
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