GFP susceptible to Tx 100

[Marcel Tanudji]

Hi all,
does anybody know if GFP is sensitive to TX-100. I wish to make cell
lysate from my transformed cells. Normally (if i'm not wrong) they use TX
100 to break the cell open.

Does anyone tried this with GFP transformed cells and does the GFP still
fluoreseced?

thanks


  =  Marcel Tanudji 						        =	
  =  Dept Biochemistry,  University of Western Australia	        =
  =  Perth, Australia	[email:mtanudji at cyllene.uwa.edu.au]             =