TritonX100

tfitzwater at NEXSTAR.COM tfitzwater at NEXSTAR.COM
Tue Dec 1 16:12:32 EST 1998


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|-------------------------+------------------------->
|                         |                         |
| Detergent               | Type                    |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | PCR inhibitory conc.    |
  |                         |
  >-------------------------|
|-------------------------+------------------------->
|                         |                         |
| Nonidet P-40            | Nonionic                |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | > 5% (v/v)              |
  |                         |
  >-------------------------|
|-------------------------+------------------------->
|                         |                         |
| Tween
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=AE20                | Nonionic                |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | > 5% (v/v)              |
  |                         |
  >-------------------------|
|-------------------------+------------------------->
|                         |                         |
| Triton=AE X-100           | Nonionic                |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | > 5% (v/v)              |
  |                         |
  >-------------------------|
|-------------------------+------------------------->
|                         |                         |
| N-octylglucoside        | Nonionic                |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | < 0.4% (w/v)            |
  |                         |
  >-------------------------|
|-------------------------+------------------------->
|                         |                         |
| Sodium desoxycholate    | Ionic                   |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | < 0.06% (w/v)           |
  |                         |
  >-------------------------|
|-------------------------+------------------------->
|                         |                         |
| Sarkosyl                | Ionic                   |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | < 0.02% (w/v)           |
  |                         |
  >-------------------------|
|-------------------------+------------------------->
|                         |                         |
| SDS                     | Ionic                   |
|                         |                         |
|-------------------------+------------------------->
  >-------------------------|
  |                         |
  | < 0.01% (w/v)           |
  |                         |
  >-------------------------|


Data reported by Robbin S. Weyant, Paul Edmonds and Bala Swaminathan 19=
90
BioTechniques 9 (3) 308-309.

Earlier report by M. A. Innes et al. (1988 PNAS 85:  9436-9440) showed =
that
0.05% Tween=AE20 and 0.05% Nonidet P-40 actually enhanced Taq DNA polym=
erase
activity.
-----
Tim Fitzwater
Prinicipal Research Associate
NeXstar Pharmaceuticals
=

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