HiFi PCR /TritonX100
rhubner at gins.uia.ac.be
Tue Dec 1 15:40:56 EST 1998
In article <3663EBBF.DBCC56E1 at pitt.edu>, rdudley+ at pitt.edu wrote:
> Markus Schneemann wrote:
... >8... snip...
> Promega has a protocol for mixing Taq and Tfl (I think) on their web page. I
That should be Tli (aka Vent); Tfl is not proofreading (Thermus derived!)...
>You can't T/A clone these products--the proofreading enzymes remove the
A-tail Taq leaves.
But if Taq is major component you CAN clone these products (unless end of
against extendase activity of Taq!)... see latest Expressions for a Q & A
on cloning long
More information about the Methods