Long PCR products

Kowthar Salim kowthar at zoo.utoronto.ca
Tue Dec 1 09:54:00 EST 1998


Hi All,

I am currently trying to amplify a region in a genomic DNA that is about 
3kb long.

I have tried to increase the extension time (3 minutes) during the PCR 
reaction.  Unfortunately, this has not yielded any PCR products.

I was wondering if anyone has any suggestions or if any references can be 
recommended.

Thank you.

Kowthar.



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