DMSO in PCR

Tsunehisa Namba drnamba at kuhp.kyoto-u.ac.jp
Wed Dec 2 02:16:59 EST 1998


Hi All

   I need to PCR GC rich sequence.  So far, I tried  various denaturing/ 
annealing temp without success.  I would like to try DMSO.
   Could anyone tell me the concentration of DMSO and other cautions for PCR?

Nam

-- 
***************************************************
Tsunehisa Namba
Department of Anesthesia, Kyoto University Hospital
Sakyo-ku, Kyoto 606-01, Japan
Fax:+81-75-752-3259
E-mail: drnamba at kuhp.kyoto-u.ac.jp



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