Oligo-hybribization-wanted

Martin Offterdinger a8803349.nospam at unet.univie.ac.at
Wed Dec 2 05:12:16 EST 1998


Hi
We are looking for a protocol for oligo hybridization to give high
yield of dsDNA. We want to subclone the resulting product and have
phosphorylated oligos. What we dont have is a protocol for hybridizing
them. I know it should involve heating above the Tm of the oligos and
cooling down slowly, but what are the experimental details. Please let
me know if you have done that successfully.
thanks
martin
Martin Offterdinger
Internal Med.I,Dept. Oncology
University of Vienna
Austria
E-Mail:a8803349.nospam at unet.univie.ac.at
(remove .nospam before mailing)



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