quantitate gene expression - which method?
william at neuro.usc.edu
Wed Dec 2 20:37:59 EST 1998
I'm interested in quantitating the expression of a particular gene within
rat heart after treatment with some hormones. But I'm faced with several
choices in regards to methods. Which one of the following is the best
(and most believable) method and why?:
1) northern blot
2) RNase protection assay
4) in situ hybridization
Thanks for any input.
William Sun, Ph.D
Metabasis Therapeutics, Inc. Phone: (619) 622-3930
9390 Towne Centre Drive FAX: (619) 622-5545
San Diego, CA 92121 Email: william at alum.mit.edu
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