Plasmid isolation from E. faecalis?

Lars Andrup andrup at internet.dk
Thu Dec 3 03:56:40 EST 1998


Dear Colleges,
We have tried several methods (several times) in an attempt to isolate plasmids from Enterococcus faecalis (pCF10, 50 kb) - without any luck. We have controls of various Bacilli containing large plasmids - hence, the detection (0.5% agarose-gels) is ok.

Any ideas?

yours

Lars

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Lars Andrup, senior researcher
National Institute of Occupational Health
Lersø Parkallé 105
DK-2100 Copenhagen, Denmark
phone: +45 3916 5223, fax: +45 3916 5201
e-mail: andrup at internet.dk
http://www.ami.dk/research/andrup
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