Ligase fidelity

Raj Mehta r-mehta at nimr.mrc.ac.uk
Thu Dec 3 13:46:09 EST 1998


I wonder if anyone has any information on the requirement of 100%
complementary ends for the various ligases available? I would like to
ligate a pool of ds DNA with random 4 base overhangs to an adaptor with
specified 4 base overhang. Which ligase is the best for maximising
ligation of 100% complementary overhangs only?

Raj



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