GFP susceptible to Tx 100

Weisong Zhou wzhou0 at pop.uky.edu
Fri Dec 4 20:02:17 EST 1998


Marcel Tanudji wrote:
> 
> Hi all,
> does anybody know if GFP is sensitive to TX-100. I wish to make cell
> lysate from my transformed cells. Normally (if i'm not wrong) they use TX
> 100 to break the cell open.
> 
> Does anyone tried this with GFP transformed cells and does the GFP still
> fluoreseced?
> 
> thanks
> 
>   =  Marcel Tanudji                                                     =
>   =  Dept Biochemistry,  University of Western Australia                =
>   =  Perth, Australia   [email:mtanudji at cyllene.uwa.edu.au]             =
I only know GFP is sensitive to methanol.



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