why no 28S RNA?

Ned Mantei mantei at cell.biol.ethz.ch
Sat Dec 5 08:59:48 EST 1998


In article <199812050855.QAA13023 at nic>, jing at NIC.HBU.EDU.CN wrote:

> Dear netters,
> 
> I have made total RNAs from earthworm for many many times, 
> but only got 18S band on agarose gel, no 28S band at all. I 
> could get 28S and 18S RNAs with other materials such as fish 
> egges and chiken liver. It doesn't like being digested by 
> RNase, because the 18S band was very sharp and small band 
> down the gel was not much . 

There are some species in which at least one of the rRNAs has a
single-stranded break. Could it be that earthworm RNA is like this, and
that the products run together with the 18S RNA?

-- 
Ned Mantei
Dept. of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland



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