why no 28S RNA?

Ned Mantei mantei at cell.biol.ethz.ch
Sat Dec 5 08:59:48 EST 1998

In article <199812050855.QAA13023 at nic>, jing at NIC.HBU.EDU.CN wrote:

> Dear netters,
> I have made total RNAs from earthworm for many many times, 
> but only got 18S band on agarose gel, no 28S band at all. I 
> could get 28S and 18S RNAs with other materials such as fish 
> egges and chiken liver. It doesn't like being digested by 
> RNase, because the 18S band was very sharp and small band 
> down the gel was not much . 

There are some species in which at least one of the rRNAs has a
single-stranded break. Could it be that earthworm RNA is like this, and
that the products run together with the 18S RNA?

Ned Mantei
Dept. of Cell Biology, Swiss Federal Institute of Technology
CH-8093 Zurich, Switzerland

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